2015, №2

сс. 17-30

Determination of Natural Aromatic Acids Using Supercritical Fluid Chromatography

2015, №2

сс. 17-30

Цитировать

D.V. Ovchinnikov, D.S. Kosyakov, N.V. Ul’yanovskii, K.G. Bogolitsyn, D.I. Falev, O.I. Pokrovskiy

Key words: supercritical fluid chromatography, SFC, natural aromatic acids

Derivatives of benzoic and cinnamic acids are secondary metabolites of plants, widely
spread in nature and possessing biological activity. It is shown that supercritical fluid
chromatography (SFC) provides a high performance separation of nine most important
representatives of this class of compounds with selectivity, which is dramatically different
from the reversed-phase high-performance liquid chromatography. The retention and
separation parameters of analytes for four chromatographic stationary phases of different
nature are compared and the effect of supercritical fluid parameters and the composition
of the mobile phase onto the chromatographic separation are analyzed. The optimum
separation can be achieved using a silica-based sorbent with 2-ethylpyridinium linked
groups. The proposed approach to determine the above mentioned compounds is based
on the combination of chromatographic separation with multiwavelength
spectrophotometric detection. It provides the detection limits in the range of 13.0—51.3
mg/L and the duration of analysis about 2.5 min. The developed approach is successfully
tested on the analysis of real objects — three different kinds of wine.

doi:10.1134/S1990793116070150