SCF-TP on-line  

2015, N2, pp. 17-30

D.V. Ovchinnikov, D.S. Kosyakov, N.V. Ulyanovskii, K.G. Bogolitsyn, D.I. Falev, O.I. Pokrovskiy

Determination of Natural Aromatic Acids Using Supercritical Fluid Chromatography

Derivatives of benzoic and cinnamic acids are secondary metabolites of plants, widely spread in nature and possessing biological activity. It is shown that supercritical fluid chromatography (SFC) provides a high performance separation of nine most important representatives of this class of compounds with selectivity, which is dramatically different from the reversed-phase high-performance liquid chromatography. The retention and separation parameters of analytes for four chromatographic stationary phases of different nature are compared and the effect of supercritical fluid parameters and the composition of the mobile phase onto the chromatographic separation are analyzed. The optimum separation can be achieved using a silica-based sorbent with 2-ethylpyridinium linked groups. The proposed approach to determine the above mentioned compounds is based on the combination of chromatographic separation with multiwavelength spectrophotometric detection. It provides the detection limits in the range of 13.051.3 mg/L and the duration of analysis about 2.5 min. The developed approach is successfully tested on the analysis of real objects three different kinds of wine.

Key words: supercritical fluid chromatography, SFC, natural aromatic acids


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