Key words: supercritical extraction, porosity, carbon dioxide, xenogenic matrix, osteoplastic materials
Multistage environmentally benign method of xenogenic bone matrix processing in
sub- and supercritical media is developed. This method provides with a fast matrix
deli pidation in supercritical carbon dioxide, deproteinization by treatment with 3%
hydrogen peroxide solution followed by washing in subcritical water for removal of
polar substances. The final treatment with supercritical carbon dioxide serves to remove
residual non-polar components. A method to control the porosity of xenogenic bone
matrix on several stages of purification is suggested. Preliminary data indicate that cell
test system based on the allogenic osteoid cell line Th-1 can be used for evaluation of
primary adhesion and cell proliferation on the surface of purified bone matrix.
doi:10.1134/S1990793115070131